Mycobacterium phage Sam22
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| Detailed Information for Phage Sam22 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Mariah Burnett |
| Year Found | 2022 |
| Location Found | Fountain Run, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.7037 N, 85.91027 W Map |
| Discovery Notes | I purified and isolated my phage by removing, picking off, an isolated plaque using a pipette. This process was done 9 times to obtain 3 different 10-fold dilutions and to obtain a single plaque. Finally, after multiple attempts to purify my dilutions we obtained turbid plaques but was able to view them because they were separated enough. |
| Naming Notes | I have a cat that I rescued from the hot heat and named him Sammy Jo "Sam" and I found him in 2022. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | My plaques had an average size of 0.5 mm. The largest plaque was 1.5 mm. They were turbid and was very hard to see until they were really separated out. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.7 x 10^9 |
| Pitt Freezer Box# | 157 |
| Pitt Freezer Box Grid# | H3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
