Arthrobacter phage SardineQuasar
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| Detailed Information for Phage SardineQuasar | |
| Discovery Information | |
| Isolation Host | Arthrobacter pascens NRRL B-1814 |
| Found By | Fardeen Kawsar |
| Year Found | 2025 |
| Location Found | Atlanta, GA United States of America |
| Finding Institution | Emory University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 33.80146 N, 84.3252 W Map |
| Discovery Notes | Soil was sampled from 3" deep in the ground next to the tree adjacent to the "Additional Parking" sign at the Emory Point Conference Center Hotel North Parking entrance behind Emory Point Apartments building 6. Sandy/dry soil, no plants, covered in dead pine needles. Phage underwent 4 rounds of purification (picking and re-plating) to obtain a homogenous phage stock. |
| Naming Notes | My name is Fardeen Kawsar and SardineQuasar sounds kinda similar. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Efficiency of lysogeny (EOL) assay yielded 0.00025% (18 out of 7.2*10^6) which falls outside the typical lysogeny range of 0.1-100%. Therefore the phage is presumed to be lytic. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Under the following plating conditions: 100µL of phage solution is sorbed with 100µL of host bacteria Arthrobacter pascens B-1814 and combined with 4mL of 0.4% PYCa top agar to make a full double-layer plate. Average plaque diameter 0.8 mm (range 0.5-1.0 mm, n=10) Plaques are clear, circular, with typically hazy edges. Sometimes a small "island" of bacteria occurs in the center of the plaque. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
