| Detailed Information for Phage Sarkar |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Justin Etter |
| Year Found | 2024 |
| Location Found | St. Louis, MO United States |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 19°C |
| GPS Coordinates | 38.6445 N, 90.3115 W Map |
| Discovery Notes | The sample was collected at a depth of 3 cm. The soil was dry but soft/spongy, located near the base of a small sapling along a paved walkway. |
| Naming Notes | Phage Sarkar is named after my High School Biology teacher, who helped grow my passion for science. I was introduced to phages in her class which helped motivate my participation in this program! |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 16, 2025 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NovaSeq X Plus |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 121 |
| Genome length (bp) | 186427 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1053 bp |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BM |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | Being Annotated (Expected completion by 12/16/2025) |
| Plaque Notes | Plaques formed by Sarkar are small and turbid with fuzzy edges. The provided image was taken after one round of purification, and the average plaque diameter was measured at about 1.5 mm. |
| Number of Genes | 342 |
| Number of tRNAs | 11 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
