Mycobacterium phage ScrappyDoo
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| Detailed Information for Phage ScrappyDoo | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Brayden Gross |
| Year Found | 2022 |
| Location Found | Somerset, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.07345 N, 84.63381 W Map |
| Discovery Notes | The soil was very dry and warm, contained many dry weeds, and lacked life. The sample was collected just below the topsoil. The ambient temperature was 24℃ (75℉). I performed 4 rounds of serial dilutions to purify my phage. |
| Naming Notes | Scooby Doo is one of my favorite cartoons and Scrappy was always temperate( very aggressive and moody) and my phage is a temperate phage, so the name fits. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | My phage can complete the lysogenic cycle or the lytic cycle because it is a temperate phage. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | My phage forms turbid plaques(4mm) with no rings. Meaning that it is a temperate phage. My phage has an average head length of 48.073nm, and a Standard deviation of 0.499217nm. my phage has an average tail length of 133.6961 and a Standard deviation of 7.703928nm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.53 x 10^9 |
| Pitt Freezer Box# | 157 |
| Pitt Freezer Box Grid# | H4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
