Mycobacterium phage ShiningRevelry
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Detailed Information for Phage ShiningRevelry | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Former names | SterileTechniq |
Found By | Jacob Escamilla, Nikhil Loordu |
Year Found | 2025 |
Location Found | Boulder, CO United States |
Finding Institution | University of Colorado Boulder |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 40.0023 N, 105.2645 W Map |
Discovery Notes | Phage SterileTechniq was isolated from soil near a parking lot beside the Kittredge West dorms at the University of Colorado-Boulder on Jan 21, 2025. The soil was found under a tree, with about 3" of snow covering the ground. The temperature was 31°F (-0.556°C) with wind gusts of 11 MPH (17.70 KPH). The phage was also isolated with the help of instructors Dr. Nancy Guild and Dr. Karen Riley, lab coordinator Megan Greening, teaching assistant Gabriella Giese, and learning assistant Cody Campos. |
Naming Notes | The name of this phage initially came from the impeccable use of sterile technique while isolating and documenting the properties of this phage. Following that, we changed the name to reflect the ShiningRevlery Pokemon pack listed in Pokemon Pocket, as it was popular at the time. It was also what we did to occupy our time while waiting for things like gel electrophoresis to run or for top agar to cool. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | The phage was lytic and able to produce a variable morphology. Due to many rounds of purification, all producing multiple morphologies, we can conclude that there is one type of phage, and the different morphologies are not due to multiple phage species. One of the morphologies produced was about 1/4-1.0mm in diameter, while the other was about 3.0mm in diameter. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Not in Pitt Archives |
Available Files | |
Plaque Picture | Download |
EM Picture | Download |