Mycobacterium phage Singhora
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Detailed Information for Phage Singhora
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDestiny Lora
Year Found2016
Location FoundBlauvelt, NY USA
Finding InstitutionDominican College of Blauvelt
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature23°C
GPS Coordinates41.0528 N, 73.9501 W Map
Discovery NotesThe day I collected my sample, I had arrived on campus bright and early. I dug about an inch into the soil in order to avoid collecting dead soil and debris. When i was an inch deep, i collected 15mL of soil. The temperature that day was around 73 degrees fahrenheit and it was also humid. What threw me off a bit was that the soil I had collected was dry and i didn't know if a virus could form in a dry area. What eased me up a bit was the fact that the soil was a dark brown color meaning that it had a high organic matter content or, in other words, the soil was useful and reliable. The next day we performed direct isolation in order to find viruses in our samples.To my luck, I had plated 500 mL of my sample instead of 1000mL but the solution was very simple! I ended plating twice, each plate containing 500mL of my sample. The next day, there was a clearing on my plate! two plaques! I succeeded, I found a virus!
Naming NotesAn acquaintance of mine has a very unique last name "Singh" and I consider my last name an odd one. "Lora" is usually seen as a first name so i decided to merge them and I got "Singhora!"
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
SubclusterK5
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesMy plaques are pretty small clearings and they are all the same size throughout.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.39x10^9
Date of SEA Lysate Titering Oct 5, 2016
Pitt Freezer Box# 35
Pitt Freezer Box Grid# H11
Available Files
Plaque PictureDownload
EM PictureDownload