Microbacterium phage Snizhenka
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| Detailed Information for Phage Snizhenka | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Snizhana Ushanly |
| Year Found | 2025 |
| Location Found | Fenton, MO United States |
| Finding Institution | St. Louis Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 38.567149 N, 90.422904 E Map |
| Discovery Notes | This project required multiple rounds of troubleshooting due to contamination during early plaque assays. Repeating the isolation process with a new environmental sample resulted in consistent plaque morphology and successful purification. These results highlight the importance of aseptic technique, repetition, and careful observation in bacteriophage discovery. |
| Naming Notes | I named this phage Snizhenka because my own name, Snizhana, means “snow” in Ukrainian. I wanted the phage’s name to reflect a small part of my identity and culture. Just as every snowflake is unique, this phage represents a unique discovery in my scientific journey. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Plaques produced by this phage were primarily cloudy, suggesting the phage may be temperate and capable of lysogeny. Cloudy plaque morphology indicates that some host cells may survive infection, consistent with potential lysogenic behavior. Further testing would be required to confirm lysogeny. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques were small (1–2 mm), circular, and clear with well-defined edges. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 3.3 × 10¹⁰ pfu/mL |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
