Mycobacterium phage Speckles
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Detailed Information for Phage Speckles
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKathryn Trentadue
Year Found2013
Location FoundHolland, MI USA
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.7833 N, 86.1 W Map
Discovery NotesPhage Speckles was isolated from moist soil obtained from 2 cm below the surface near a sidewalk in a high-traffic area. The soil was taken from under the concrete steps leading up to a residence hall (Dykstra).
Naming Notes"Speckles" refers to the appearance of the plaques produced by this phage in the bacterial lawn.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesPhage Speckles produces a bullseye plaque morphology, with a turbid center (occasionally with a small clear dot in the very center) surrounded by a clear ring, surrounded by a turbid ring. The size is normally between 4 mm and 7 mm, with an average size of about 4.9 mm.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5.6x10^10
Pitt Freezer Box# 5
Pitt Freezer Box Grid# B12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload