Mycobacterium phage Spork
Spork Plaque Picture
Spork EM Picture
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Detailed Information for Phage Spork
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMaggie Cook
Year Found2016
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates36.9871 N, 86.4543 E Map
Discovery NotesDate Found: 9-20-16
Temperature: 87°F/31°C
Depth of Digging: 1-1 1/2 inches deep
Moisture Content: Moist
Time of Collection: 13:27
Other notes: An area of low foot traffic area, with high exposure to direct sunlight. In between a plant bed containing bushes and the edge of a lawn, near a waste receptacle with some organic waste near site. Rain had occurred very recently.
Naming NotesUsed a plastic spork to dig up the sample containing my phage.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Lysogeny NotesThe plaques were lytic and clear with 24 and 48 hour incubation periods at 37°C
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaques were medium sized, with a diameter of 0.5 mm. They were not normally clustered.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2016WEKUsporkMC
SEA Lysate Titer 2.3 x 10^8 pfu/mL
Date of SEA Lysate Titering Oct 20, 2016
Pitt Freezer Box# 37
Pitt Freezer Box Grid# D5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload