Mycobacterium phage Squiggles
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| Detailed Information for Phage Squiggles | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Mikayla Jellico and Tyler Kapolnek |
| Year Found | 2025 |
| Location Found | Longmont, CO United States |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.135654 N, 105.162391 W Map |
| Discovery Notes | Enriched soil was collected from a fruit and vegetable garden in early January. Garden has a history of containing crops of watermelon, cucumbers, yellow corn, green-leaf lettuce, orange carrots, jack-o'-lantern pumpkins, green onions, cherry tomatoes. Pumpkins have been the most prominent in this garden. Squash pumpkins (jack be little, sugar, and white queen pumpkin varieties) from the fall season of 2024 were mixed with the soil prior to collection. |
| Naming Notes | Squiggles is a name representative of the slightly disproportional capsid head diameter and tail length (compared to average), as well as the variable behavior exhibited during infection of Mycobacterium smegmatis mc^2155. The behavior that results in different plaque sizes (most noted 4mm-6mm) opens the conclusion that the behavior of this mycobacteriophage is not linear nor predictable, but rather resembles a "squiggle", which cannot be easily labeled objectively per infection. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Average tail length (referencing 9 EM images that show the images of 5 individual mycobacteriophage of the same species): 127.9 nm Average capsid diameter (referencing 9 EM images that show the images of 5 individual mycobacteriophage of the same species): 66.9 nm Description: Relatively shorter tail length compared to average tail length of Siphoviridae family mycobacteriophage. Tail is long and is able to bend. Capsid head size is around average size across all bacteriophage species. Capsid head size is larger than average across observed mycobacteriophage within the Siphoviridae family. Capsid head appears icosahedral. Tail fibers are faintly present in EM images, which suggests a Siphoviridae morphology rather than a Myoviridae morphology. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |