Mycobacterium phage Stawickiterium
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Detailed Information for Phage Stawickiterium
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByElena Baggett
Year Found2022
Location FoundBowling Green, KY United States of America
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.950556 N, 86.466389 W Map
Discovery NotesThis sample was collected in a moist dirt patch in Bowling Green, KY. It underwent 4 serial dilutions after being enriched. Plaque morphology was consistent in each, with the most countable plate resulting in a titer of 1.0 x 10^7 pfu/mL. Then, a ten plate lysate was completed, resulting in a high titer of 6.1 x 10^8 pfu/mL. Once the ten plate lysate was complete and calculated, the phage were then sent to an electron microscope to determine morphology. The average tail length, when measured, was 131.071 nm while the average head diameter was 47.227 nm. When the DNA was isolated, it was found to have a concentration of 60.8 ng/μL. Once the DNA was isolated, it was then subjected to gel electrophoresis and many restriction digest enzymes that revealed this phage to have a rich GC genome.
Naming NotesThe name came from Nikolas Stawicki's last name, the person who worked with this phage in the lab.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaques were turbid and not bountiful at all. Very small in diameter, with the average being .15cm.
MorphotypeMyoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6.1 x 10^8
Pitt Freezer Box# 157
Pitt Freezer Box Grid# H7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload