Arthrobacter phage Stingy
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| Detailed Information for Phage Stingy | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis NRRL B-4425 |
| Found By | Ella Stoppenbrink |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 45.41941 N, 75.66796 W Map |
| Discovery Notes | Stingy was discovered in soil in Wiggins Pvt. Community Garden in Ottawa, ON, Canada. Soil sample was collected from 1cm below the surface and was soft and moist. The ambient temperature was 23˚C. Genomic DNA was sequenced on a ONT Minion, R10.4.1 chemistry using rapid barcoding V14 and PCR amplification. The sequence was assembled into one contig of 57,382bp with 78% query cover, 81.07% identity to Inked (AU7). This sequence appears to be nearly complete based on its similarity to Inked which is a similar size to the longest contig. |
| Naming Notes | During the purification process, this phage did not infect the host on two separate rounds of plaque assays and because of this stubborn behaviour, it was named Stingy meaning unwilling to give or share. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaque sizes are slightly un-uniform with both small and larger circular plaques. The plaques are clear and well defined. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |