Mycobacterium phage Swinger
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| Detailed Information for Phage Swinger | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Liz Swinger |
| Year Found | 2022 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.98873 N, 86.44495 W Map |
| Discovery Notes | Soil sample was isolated from a suburban, highly populated area, then introduced to host in an enrichment. The enrichment went through 8 serial dilutions before purified to show consistent turbid 2mm plaques. One plate lysate did not yield a high enough titer, so the 10 plate lysate was used for a titer of 3.0 x 10^9 pfu/mL. DNA purification/isolation yielded 100.8 ng/uL of DNA. Phage tails measured at an average of about 140 nm. Gel electrophoresis showed cutting at above 10.0kb, but HaeIII showed cutting at 1.5kb and several times more showing a smeared result on labeled digest. |
| Naming Notes | I have always taken pride and humor in my last name, but I have nothing but pride regarding the phage I have found and the work it took to get there. I have named my phage after my last name with that in mind. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Turbid plaques measured at 2.0mm, no halo was seen. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3.0 x 10^9 |
| Pitt Freezer Box# | 157 |
| Pitt Freezer Box Grid# | H8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
