Mycobacterium phage Sylvie12
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| Detailed Information for Phage Sylvie12 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Elizabeth Barnett |
| Year Found | 2025 |
| Location Found | Clarkson, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.48238 N, 86.06054 W Map |
| Discovery Notes | Sample Collection Date: September 1st, 2025 It was collected outside a tree beside my house. The temperature was 83°F and it was very sunny. It had not rained for a while, so the soil was dry and clumped together. It was taken from around 2 centimeters in the ground. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | My phage produced turbid, medium-sized plaque phenotypes, however, there were variations within the size of the plaques produced. In order to determine the mean and the standard deviation within the large and small plaque samples, I measured my plates on October 14th, 2025. Large Plaque Sample: Mean: 0.4 cm Standard Deviation: 0.08944 Small Plaque Sample: Mean: 0.37 cm Standard Deviation: 0.55758 Overtime, the plaques that were produced within my plates began to grow smaller. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 2.04 x 10^8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
