Mycobacterium phage Tesa
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| Detailed Information for Phage Tesa | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Samuel Terrell |
| Year Found | 2021 |
| Location Found | OWENSBORO, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 37.779426 N, 87.130683 W Map |
| Discovery Notes | This phage was originally collected in Owensboro Kentucky from the mud of the Ohio river. The soil sample was then filtered and enriched then serial diluted to isolate sample down to one phage. Following this, a high titer stock was created from a 10-plate lysate where it was then exposed to restriction enzymes to then separate the cuts via gel electrolysis. |
| Naming Notes | The name was chosen by combining the first two letters of the researchers first and last name. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | The lysogeny of the Tesa phage was what we expected. It grew well in laboratory conditions and worked well with all of the procedures we used. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques of the Tesa phage normally ranged from .6cm to .8cm. The plaques had a clear center with a turbid halo. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.9x10^9 |
| Pitt Freezer Box# | 138 |
| Pitt Freezer Box Grid# | A5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
