Arthrobacter phage Thief
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Detailed Information for Phage Thief | |
Discovery Information | |
Isolation Host | Arthrobacter sp. ATCC 21022 |
Duplicates of this Phage | Jenesis2 |
Found By | Christina Gaw |
Year Found | 2016 |
Location Found | Baden, PA USA |
Finding Institution | Baylor University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 40.654299 N, 80.222683 W Map |
Discovery Notes | This phage was adopted from Alec Ingros. The sample it was contained in was collected on 8/9/16 at 6:30 PM under a tree near a soccer field. The temperature was 77 degrees Fahrenheit. It contained 8.3% sand, 4.3% silt, and 20.8% clay. The pH of the soil was 5.7. The percentage of the soil with water is 31.4% "The soil was collected 3-4 inches under the top soil, vegetation was present in the sample, it was raining while soil was collected." The high titer is from mixing two lysates together that had 317 microliters of lysate and 267 microliters of lysate. The EM image and Digest images were collected from lysate with a titer of 4.0*10^7 The DNA gel electrophoresis results were negative; therefore the digest image was not included in this entry. |
Naming Notes | The name was from the adoption of the phage. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | The plaques are small and lytic. The plaques are approximately 0.5 millimeters in size. A collection of plaques can be found in the top left corner of the plate. This was where the observations were from. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | 6.00*10^11 |
Date of SEA Lysate Titering | Dec 2, 2016 |
Pitt Freezer Box# | 51 |
Pitt Freezer Box Grid# | F7 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |