Mycobacterium phage TopPatt
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage TopPatt | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Caitlyn Patterson |
Year Found | 2023 |
Location Found | Glasgow, KY United States of America |
Finding Institution | Western Kentucky University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 30°C |
GPS Coordinates | 37.0175 N, 85.966389 W Map |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Lysogeny Notes | During spot plating protocol, a mesa formation was present in the 10^0 clearing when incubated at 37℃. This formation is evidence that Mycobacterium smegmatis is being induced as a lysogen. This spot formation was used to produce a T-Streak plate. A colony from this T-Streak plate was inoculated at 37℃ until the liquid media was turbid. This inoculation was used to set up a second set of spot plates. The lysogen plated at 37℃ showed no growth which verified that a lysogen was isolated. The lysogen plated 30℃ presented with plaques, indicating spontaneous conversion into the lytic cycle. |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | Halos present in plaques |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Not in Pitt Archives |
SEA Lysate Titer | 9.5 X 10^8 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |