Mycobacterium phage TopPatt
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Detailed Information for Phage TopPatt
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCaitlyn Patterson
Year Found2023
Location FoundGlasgow, KY United States of America
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates37.0175 N, 85.966389 W Map
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Lysogeny NotesDuring spot plating protocol, a mesa formation was present in the 10^0 clearing when incubated at 37℃. This formation is evidence that Mycobacterium smegmatis is being induced as a lysogen. This spot formation was used to produce a T-Streak plate. A colony from this T-Streak plate was inoculated at 37℃ until the liquid media was turbid. This inoculation was used to set up a second set of spot plates. The lysogen plated at 37℃ showed no growth which verified that a lysogen was isolated. The lysogen plated 30℃ presented with plaques, indicating spontaneous conversion into the lytic cycle.
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesHalos present in plaques
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 9.5 X 10^8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload