Streptomyces phage Toph
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| Detailed Information for Phage Toph | |
| Discovery Information | |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Jimmy Nguyen & Sarvesh Neupane |
| Year Found | 2018 |
| Location Found | Catonsville, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 39.252411 N, 76.708629 W Map |
| Discovery Notes | September 10, 2018 at ~12:55-1:00 PM. Collected next to a dead tree beyond a tunnel on UMBC campus. Dirt was dug about half an inch to 1 inch deep. Weather was 66 degrees Fahrenheit. Cloudy, Drizzling 99% humidity Soil was damp. |
| Naming Notes | Named after children's cartoon character, Toph Beifong. Name is written as: 北方拓芙 meaning "expanding lotus" in Mandarin. Can be seen as a pun for "tough." "Toff" is British-slang for upper class. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | A "mesa" formed on a spot test for S. scabiei in 24-48 hours of incubation, but lysogeny tests were performed to suggest that Toph may actually be virulent. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques were about 1 mm, clear, roundish, with uneven, jagged edges after 24-28 hours at 28 degrees C on S.scabiei |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.3x10^9 pfu/mL |
| Pitt Freezer Box# | 84 |
| Pitt Freezer Box Grid# | A12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
