Mycobacterium phage Toreador
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| Detailed Information for Phage Toreador | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Reagan Bushok, Michael Heraty |
| Year Found | 2021 |
| Location Found | West Lafayette, IN United States |
| Finding Institution | Purdue University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.42166 N, 86.91404 W Map |
| Discovery Notes | Date of soil collection: August 30, 2021 at 4:17 pm Location: Purdue University Horticulture Garden, directly underneath/around the roots of the Toreador Cockscomb flowers. Weather Conditions: 81 degrees Fahrenheit, 15% precipitation, 68% humidity, according to Google Weather at 4:17 pm. Overcast. Soil Conditions: Collected dark and slightly damp/moist soil near the roots of the flowers. This may have been due to previous precipitation or watering of flowers. Collection Methods: Soil taken ~1 inch from surface, directly underneath mulch. White, plastic spoon used to scoop dirt into plastic bag. Working on Phage but did not originally discover it: Benjamin Goncher, Hayden Tippmann |
| Naming Notes | Named after the Toreador Cockscomb flower (Celosia argentea ‘Toreador’), as this phage was collected underneath the roots of this flower. Collected at the Horticulture Garden of Purdue University. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Because the plaques have cloudy edges, it can be hypothesized that this phage is lysogenic or temperate. However, many agar plates were also completely lysed, so this phage could also be lytic. More research needs to be done to confirm. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Across agar plates, the plaques were similar in small diameter/size, and visual characteristics - the plaques were clear, circular shapes with slightly cloudy edges. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.9 x 10^9 |
| Pitt Freezer Box# | 138 |
| Pitt Freezer Box Grid# | C12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
