Mycobacterium phage Tris
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| Detailed Information for Phage Tris | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Joas Alfajardo |
| Year Found | 2014 |
| Location Found | Providence, RI USA |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.830211 N, 71.402508 W Map |
| Discovery Notes | The phage was isolated from soil in front of the Andrew Commons cafeteria building. It rained the previous night and the surrounding area was on a higher elevation, making the area where I harvested a prime location for phage to accumulate based on the water run-off. The approximate temperature was 75 degrees Fahrenheit and the depth of the sample dug was around 3/4ths of an inch deep. Vegetation was also present about a foot away from the sample site. |
| Naming Notes | The name comes from the way things always come in threes, forming the prefix tri-. The s was added to make the name sound more like a name and to fulfill the naming requirements. Coincidentally, Tris is also a chemical compound often used in biochemistry and molecular biology labs. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques are generally 1mm in diameter and clear; it's most likely a lytic bacteriophage. There was slight contamination in the plates during some purification rounds. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.03*10^11 |
| Date of SEA Lysate Titering | Oct 31, 2014 |
| Pitt Freezer Box# | 11 |
| Pitt Freezer Box Grid# | G1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
