Streptomyces phage Tubberson
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| Detailed Information for Phage Tubberson | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Jennifer Pham, Linda Quach, Megan Pham |
| Year Found | 2024 |
| Location Found | Burtonsville, MD United States of America |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.10035 N, 76.9289 W Map |
| Discovery Notes | The soil sample was collected on 9/4/24 from an area shaded by trees; it was partly cloudy that day. The soil was dark brown, fine, and moist. Direct Isolation procedure was performed on the soil sample, which then produced successful plaques! This soil sample was donated from Megan Pham (TA) to Jennifer Pham and Linda Quach. |
| Naming Notes | We originally wanted to name our phage Tubby, but we realized that the name was already taken. So, in honor of Tubby, Tubberson was born to carry on Tubby's legacy. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 14, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 5195 |
| Genome length (bp) | 39028 |
| Character of genome ends | Circularly Permuted |
| GC Content | 71.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BC |
| Subcluster | BC1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Patch assays confirmed that bacterial colonies infected by Tubberson were lysogens, implying Tubberson is likely a temperate phage. |
| Other Cluster Members | |
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Clear, large, round plaques with slightly cloudy periphery. Measured diameter of 10 mm after 48 hours with S. mirabilis in the incubator. |
| Morphotype | Siphoviridae |
| Number of Genes | 53 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewTubberson_1 Tubberson_2 Tubberson_3 Tubberson_4 Tubberson_5 Tubberson_6 Tubberson_7 Tubberson_8 Tubberson_9 Tubberson_10 Tubberson_11 Tubberson_12 Tubberson_13 Tubberson_14 Tubberson_15 Tubberson_16 Tubberson_17 Tubberson_18 Tubberson_19 Tubberson_20 Tubberson_21 Tubberson_22 Tubberson_23 Tubberson_24 Tubberson_25 Tubberson_26 Tubberson_27 Tubberson_28 Tubberson_29 Tubberson_30 Tubberson_31 Tubberson_32 Tubberson_33 Tubberson_34 Tubberson_35 Tubberson_36 Tubberson_37 Tubberson_38 Tubberson_39 Tubberson_40 Tubberson_41 Tubberson_42 Tubberson_43 Tubberson_44 Tubberson_45 Tubberson_46 Tubberson_47 Tubberson_48 Tubberson_49 Tubberson_50 Tubberson_51 Tubberson_52 Tubberson_53 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1.31 * 10^10 pfu/mL |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
