Streptomyces phage Tubberson
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| Detailed Information for Phage Tubberson | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Jennifer Pham, Linda Quach, Megan Pham |
| Year Found | 2024 |
| Location Found | Burtonsville, MD United States of America |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.10035 N, 76.9289 W Map |
| Discovery Notes | The soil sample was collected on 9/4/24 from an area shaded by trees; it was partly cloudy that day. The soil was dark brown, fine, and moist. Direct Isolation procedure was performed on the soil sample, which then produced successful plaques! This soil sample was donated from Megan Pham (TA) to Jennifer Pham and Linda Quach. |
| Naming Notes | We originally wanted to name our phage Tubby, but we realized that the name was already taken. So, in honor of Tubby, Tubberson was born to carry on Tubby's legacy. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 14, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 5195 |
| Genome length (bp) | 39028 |
| Character of genome ends | Circularly Permuted |
| GC Content | 71.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BC |
| Subcluster | BC1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Patch assays confirmed that bacterial colonies infected by Tubberson were lysogens, implying Tubberson is likely a temperate phage. |
| Other Cluster Members | |
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Clear, large, round plaques with slightly cloudy periphery. Measured diameter of 10 mm after 48 hours with S. mirabilis in the incubator. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1.31 * 10^10 pfu/mL |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
