Streptomyces phage Twist
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| Detailed Information for Phage Twist | |
| Discovery Information | |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Deven Patel, Amirah Stock |
| Year Found | 2025 |
| Location Found | Orchard Park, NY United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 42.771173 N, 78.788195 W Map |
| Discovery Notes | Phage was discovered with soil samples from High Mark Stadium in Orchard Park, New York, home to the Buffalo Bills. Conditions of environment at the time of collection were warm yet moist, but not humid. Temperature was roughly 79°F, collected on August 15, 2025, at approximately 8:35PM EST. Sample itself was obtained roughly 2 inches below the surface. |
| Naming Notes | Our shared love for Costco's Twist Ice Cream. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | BI |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | No observable mesa formations or turbid growth after 120 to 168 hours of incubation at 30ºC on S. griseus. Across all dilutions, plaques remained consistently clear, suggesting that Twist is of a virulent nature. No observable growth was noticed across any dilution of lytic regions, further suggesting Twist follows the lytic cycle. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Twist forms clear, circular plaques on Streptomyces griseus, averaging approximately 3.89 mm in diameter with sharp and defined margins. On S. mirabilis, plaques appear turbid with hazy edges. Twist presented physical morphological characteristics of a 78x73 nm capsid size, and approximately 330x12 nm non-contractile tail size. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1x10^8 pfu/mL |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
