| Detailed Information for Phage Vash | |
| Discovery Information | |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Jacob Kelly |
| Year Found | 2018 |
| Location Found | Burleson, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.5421 N, 97.3208 W Map |
| Discovery Notes | Collected @ night time across from an old garden, below a tree and above where many of my childhood pets are buried. About 39 degrees F. Light brown, dry soil with a small amount of plant matter. |
| Naming Notes | It was named for Vash: the Humanoid Typhoon from the series Trigun. This was due to the clear round plaques my phage was able to produce, similar to how he turned an entire town into a round crater as well as blasting a large crater into the moon. He tends to get into trouble often, but with little effort, everything ends up working out pretty well for him. This reminds me of how little effort I seemed to have to put into my phage in order to get it to work out so well. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 4, 2018 |
| Sequencing Facility | University of North Texas |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 357 |
| Genome length (bp) | 41578 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCCGGCCCCA |
| GC Content | 62.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BB |
| Subcluster | BB1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | Numerous, round, clear plaques 1 mm or less in size. Spot Test shows slight regrowth at higher concentrations. |
| Morphotype | Siphoviridae |
| Number of Genes | 54 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewVash_1 Vash_2 Vash_3 Vash_4 Vash_5 Vash_6 Vash_7 Vash_8 Vash_9 Vash_10 Vash_11 Vash_12 Vash_13 Vash_14 Vash_15 Vash_16 Vash_17 Vash_18 Vash_19 Vash_20 Vash_21 Vash_22 Vash_23 Vash_24 Vash_25 Vash_26 Vash_27 Vash_28 Vash_29 Vash_30 Vash_31 Vash_32 Vash_33 Vash_34 Vash_35 Vash_36 Vash_37 Vash_38 Vash_39 Vash_40 Vash_41 Vash_42 Vash_43 Vash_44 Vash_45 Vash_46 Vash_47 Vash_48 Vash_49 Vash_50 Vash_51 Vash_52 Vash_53 Vash_54 — tRNA Vash_55 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK450421 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 75 |
| Pitt Freezer Box Grid# | F12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
