Mycobacterium phage Whiplash
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| Detailed Information for Phage Whiplash | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Samantha Patten & Kaitlyn Tuller |
| Year Found | 2024 |
| Location Found | Boulder, CO United States of America |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.01077 N, 105.25613 W Map |
| Discovery Notes | Soil sample collected at 7:11 pm on 9/3/2024. The temperature was 26°C. The soil sample was obtained from a patch of grass next to the Boulder creek. |
| Naming Notes | Phage was named Whiplash because of the varying plaque sizes that was thought to be different phages, which resulted in setbacks in the experiment schedule, to re-perform purification thus the phage was named Whiplash. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Cloudy plaques of ~2.5mm in diameter were frequently shown. This phage occasionally showed varying plaque morphology where the plaques were clear and ~4 mm in diameter, even after purification was performed again. Several rounds of purification were completed, thus it is thought that one phage was isolated, but this phage produces variable plaques. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3.8E9 pfu/mL |
| Pitt Freezer Box# | 210 |
| Pitt Freezer Box Grid# | E6 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |