Mycobacterium phage Whiplash
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Detailed Information for Phage Whiplash | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Samantha Patten & Kaitlyn Tuller |
Year Found | 2024 |
Location Found | Boulder, CO United States of America |
Finding Institution | University of Colorado Boulder |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 37°C |
GPS Coordinates | 40.01077 N, 105.25613 W Map |
Discovery Notes | Soil sample collected at 7:11 pm on 9/3/2024. The temperature was 26°C. The soil sample was obtained from a patch of grass next to the Boulder creek. |
Naming Notes | Phage was named Whiplash because of the varying plaque sizes that was thought to be different phages, which resulted in setbacks in the experiment schedule, to re-perform purification thus the phage was named Whiplash. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | Cloudy plaques of ~2.5mm in diameter were frequently shown. This phage occasionally showed varying plaque morphology where the plaques were clear and ~4 mm in diameter, even after purification was performed again. Several rounds of purification were completed, thus it is thought that one phage was isolated, but this phage produces variable plaques. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Not in Pitt Archives |
SEA Lysate Titer | 3.8E9 pfu/mL |
Available Files | |
Plaque Picture | Download |
EM Picture | Download |