Mycobacterium phage Willica
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| Detailed Information for Phage Willica | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | John (Jack) Gillis |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 18°C |
| GPS Coordinates | 45.390433 N, 75.638713 W Map |
| Discovery Notes | The phage LisanAlWMT was adopted from Jack Gillis, a member of the lab who originally isolated it. Jack collected his soil sample from a garden bed in his backyard, where the soil was loamy and nutrient-rich, providing favorable conditions for bacteriophage discovery. The soil sample supported the isolation of phages capable of infecting Mycobacterium smegmatis. Although I did not perform the initial isolation, I continued the characterization and imaging process for this phage as part of my research. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | I observed numerous small to medium-sized plaques scattered across the Mycobacterium smegmatis 7H10 plate. Most plaques were clear with well-defined edges, indicating strong lytic activity. Several plaques exhibited subtle concentric rings, resembling a bullseye pattern, which could suggest multiple rounds of infection or lysis within the bacterial lawn. There was no visible filling-in of the plaques, and I did not observe any obvious spontaneous clear plaque mutants. Based on these observations, it appears that the phage I amplified maintains consistent lytic behavior. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |