Mycobacterium phage Willica
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| Detailed Information for Phage Willica | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Williams Thottungal |
| Year Found | 2025 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 45.390433 N, 75.638713 W Map |
| Discovery Notes | Isolated from soil found at local vegetable garden. Genomic DNA from Willica was sequenced using the Oxford Nanopore Technologies MinION platform with R10.4.1 flow cell chemistry and rapid barcoding (V14). The longest assembled contig was 68,821 bp, aligning to phage PhatLouie (Cluster B1) with 99% query coverage and 99.59% nucleotide identity. These data support classification of Willica within Cluster B1. |
| Naming Notes | The name Willica comes the saying "when there is a will there's a way". Time and time again in the lab throughout the process of isolating and characterizing the phage, I faced setbacks but through constant will and persistence in the scientific method, I was able to find the way to isolating and characterizing my bacteriophage, Willica. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Numerous small to medium, clear plaques with sharp edges were observed, consistent with strong lytic activity. Some plaques exhibited subtle concentric “bullseye” rings, suggesting multiple infection cycles. No filling-in or spontaneous clear-plaque mutants were noted. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
