Mycobacterium phage Wimpy
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| Detailed Information for Phage Wimpy | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | John Mason, Ben Winski |
| Year Found | 2010 |
| Location Found | West Lafayette, IN USA |
| Finding Institution | Purdue University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.425283 N, 86.93055 W Map |
| Discovery Notes | Mud in a runoff drain, near a highway. Temperature around 72°F. The environmental sample effectually underwent two enrichments, the first in the sense described in the lab manual, and the second as the filtered sample dilutions sat in 4.5 ml M. smegmatis culture over a weekend due to dubious media. Even then, plaques were only observed on the 10^0 dilution plate. Re amplified and purified Jenna Ischinger, Samuel Spence, Megan Walker, Quintin Walter, and Brice Wuthrich. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques were exceptionally small, so their diameter and turbidity were difficult to analyze. The plaques had a diameter of less than 1 mm, and only appeared after roughly 48 hours on a plate. The turbidity appeared clear. There was little to no variation in the plaque size, and they did not noticeably grow after observed. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | Kari Clase |
| SEA Lysate Titer | >4e9 |
| Date of SEA Lysate Titering | Dec 7, 2010 |
| Pitt Freezer Box# | 16 |
| Pitt Freezer Box Grid# | 9 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |