Mycobacterium phage Woat22
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Detailed Information for Phage Woat22
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRachel Brown
Year Found2021
Location FoundPhiladelphia, PA United States
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates39.9931 N, 75.237 W Map
Discovery NotesWoat22 was found in front of a bush next to the blue emergency light on Overbrook Ave, behind Bellarmine Hall on Saint Joseph’s University campus. Woat22 was found on February 11, 2021 at approximately 1:34 PM. The weather was cold and cloudy and it was the day after snowfall. The humidity was 84% and the ambient temperature was 0 degrees Celsius. Woat22 was collected from moist and rocky soil sample that was collected at a depth of 10.5 cm.

Woat22 underwent four rounds of purification. The MTL titer was 1.3x10^10 and the HTL titer was 2.1x10^11. After measuring seven phages, the average capsid diameter was 60.33 nm and the average tail length was 336.56 nm.

Likely belongs to B1 cluster based on cluster-specific PCR.
Naming NotesIt is an acronym for “Worst of All Time" and a play on words from the original, better-known acronym “Goat" meaning “Greatest of All Time”. It is a joke between a friend and I. 22 was added to the end of the name as it my number on my soccer jersey.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe phage produced clear, medium sized plaques which had an average range of 3 mm in diameter. The plaques were round with defined edges and formed about 24 hours after incubation at 37 degrees celsius.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload