Mycobacterium phage Woat22
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| Detailed Information for Phage Woat22 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rachel Brown |
| Year Found | 2021 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 39.9931 N, 75.237 W Map |
| Discovery Notes | Woat22 was found in front of a bush next to the blue emergency light on Overbrook Ave, behind Bellarmine Hall on Saint Joseph’s University campus. Woat22 was found on February 11, 2021 at approximately 1:34 PM. The weather was cold and cloudy and it was the day after snowfall. The humidity was 84% and the ambient temperature was 0 degrees Celsius. Woat22 was collected from moist and rocky soil sample that was collected at a depth of 10.5 cm. Woat22 underwent four rounds of purification. The MTL titer was 1.3x10^10 and the HTL titer was 2.1x10^11. After measuring seven phages, the average capsid diameter was 60.33 nm and the average tail length was 336.56 nm. Likely belongs to B1 cluster based on cluster-specific PCR. |
| Naming Notes | It is an acronym for “Worst of All Time" and a play on words from the original, better-known acronym “Goat" meaning “Greatest of All Time”. It is a joke between a friend and I. 22 was added to the end of the name as it my number on my soccer jersey. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The phage produced clear, medium sized plaques which had an average range of 3 mm in diameter. The plaques were round with defined edges and formed about 24 hours after incubation at 37 degrees celsius. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 130 |
| Pitt Freezer Box Grid# | A5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
