Mycobacterium phage Wolphred
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Detailed Information for Phage Wolphred | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Former names | Phred |
Found By | Brenna Doyle |
Year Found | 2016 |
Location Found | Philadelphia, PA USA |
Finding Institution | Drexel University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 39.959232 N, 75.188883 W Map |
Discovery Notes | Date: 9/19/16 Time: 2:58 pm Depth: 2.55 in = 6.48 cm Approx. Temp.: 71 degrees Farenheit General Location: Summer Winter Community Garden Soil sample was collected along a footpath located in a garden within an urban setting. There was a light rain at the time of collection. The soil, while moist at the top, was somewhat dry once dug up. The soil was also somewhat clumpy. Being in a garden, the collection site was surrounded by a variety of plants and there were also a few chickens located nearby. |
Naming Notes | In the cartoon series SpongeBob Squarepants, there is a sort of a running gag. There is a fish who can be quoted to yell out "My leg!" in the face of disaster in different episodes. This fish's name is Fred. Fred is the inspiration for my phage name. I simply switched out the F for a Ph. |
Sequencing Information | |
Sequencing Complete? | No |
Sequencing Facility | University of Pittsburgh Genomics and Proteomics Core Laboratories |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | My plaques are generally on the larger side, with an average diameter of 0.5 cm. They have well-defined borders and are circular in shape. They all have low turbidity. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | 5.1x10^10 pfu/ml |
Date of SEA Lysate Titering | Oct 31, 2016 |
Pitt Freezer Box# | 38 |
Pitt Freezer Box Grid# | F11 |
Available Files | |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |