Mycobacterium phage Wolphred
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Detailed Information for Phage Wolphred
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesPhred
Found ByBrenna Doyle
Year Found2016
Location FoundPhiladelphia, PA USA
Finding InstitutionDrexel University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates39.959232 N, 75.188883 W Map
Discovery NotesDate: 9/19/16

Time: 2:58 pm

Depth: 2.55 in = 6.48 cm

Approx. Temp.: 71 degrees Farenheit

General Location: Summer Winter Community Garden

Soil sample was collected along a footpath located in a garden within an urban setting. There was a light rain at the time of collection. The soil, while moist at the top, was somewhat dry once dug up. The soil was also somewhat clumpy. Being in a garden, the collection site was surrounded by a variety of plants and there were also a few chickens located nearby.
Naming NotesIn the cartoon series SpongeBob Squarepants, there is a sort of a running gag. There is a fish who can be quoted to yell out "My leg!" in the face of disaster in different episodes. This fish's name is Fred. Fred is the inspiration for my phage name. I simply switched out the F for a Ph.
Sequencing Information
Sequencing Complete?No
Sequencing FacilityUniversity of Pittsburgh Genomics and Proteomics Core Laboratories
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesMy plaques are generally on the larger side, with an average diameter of 0.5 cm. They have well-defined borders and are circular in shape. They all have low turbidity.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5.1x10^10 pfu/ml
Date of SEA Lysate Titering Oct 31, 2016
Pitt Freezer Box# 38
Pitt Freezer Box Grid# F11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload