Mycobacterium phage WonderFalls
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| Detailed Information for Phage WonderFalls | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Molly Diamond |
| Year Found | 2012 |
| Location Found | Pullman, WA USA |
| Finding Institution | Washington State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 46.730094 N, 117.178467 W Map |
| Discovery Notes | Collected 9-11-12. Air temperature 63?F. Sample taken from just beneath the surface of a gravely area in Downtown Pullman. Soil was by a path and manmade waterfall. Ground was moist. |
| Naming Notes | I first nicknamed my phage "falls" because I found them near a manmade waterfall. One day in lab I accidentally froze my spot test. I panicked and redid the spot test, but I also put the frozen spot test in the incubator, just in case. I thought if the frozen plate produced plaques, it would be a wonder phage. Plaques grew and I was very proud, so they're my wonder plaques. Wonder Falls was also the name of an obscure TV show my mother and I used to watch, so it is partially an homage. I think the name fits. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | My morphology changed from the medium titer lysate to the high titer lysate. My plaques are round and clear. They produce both large and small plaques. At first they just have shadows of halos, but if you leave them out they grow to bullseyes. After the high titer lysate they no longer grew halos. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2012WASUwonderfallsMMD |
| SEA Lysate Titer | 1.55 x 10^9 |
| Date of SEA Lysate Titering | Nov 13, 2012 |
| Pitt Freezer Box# | 42 |
| Pitt Freezer Box Grid# | 50 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
