| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Annie Tang and Hanna Jin |
| Year Found | 2023 |
| Location Found | Golansville, VA United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 37.980611 N, 77.491896 W Map |
| Discovery Notes | Soil was dry and dusty on surface, slighlty below soil surface contained slightly dry soil with larger clumps. Soil still mostly dry, light to medium brown in coloration, very few debris in sample. Sample taken from the bottom of a large deciduous tree. Temperature on 9/4 when sample was taken was around 82 degrees fahrenheit. Humidity that day was between 65% to 75%. Soil sample was processed on 9/19. |
| Naming Notes | As the phage was found from a soil sample from Golansville, VA, we decided to stick with a name that started with a "G" and "V", resulting in "GoblinVoyage". |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 19, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1166 |
| Genome length (bp) | 43540 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGCCCT |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BI |
| Subcluster | BI2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | No lysogens noted after over a week of incubation |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | On Streptomyces scabiei and Streptomyces mirabilis, plaques were between 0.5 and 3mm in diameter. S. scabiei plaques were turbid and S. mirabilis plaques were clear. Both had clean margins. |
| Morphotype | Siphoviridae |
| Number of Genes | 59 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP725412 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
171 |
| Pitt Freezer Box Grid# |
C2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
