Mycobacterium phage Nemain
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| Detailed Information for Phage Nemain | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Natasha George |
| Year Found | 2016 |
| Location Found | Santa Cruz, CA USA |
| Finding Institution | University of California, Santa Cruz |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | Unavailable |
| Discovery Notes | Soil was collected from compost at the Chadwick Garden on the UCSC campus. Samples were then incubated with M.smegmatis for 1-2 days, and filtered media was spotted on a lawn of M. smegmatis to detect phage by plaque presence. Homogenous plaques were achieved through repeatedly picking plaques and plating dilutions with M. smegmatis. |
| Naming Notes | Nemain is a figure in Irish mythology associated with war and nightmare. In the Tain Bo Cuailnge, she shows up the night before the battle to sow terror and chaos in the sleeping army. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | F |
| Subcluster | F1 |
| Cluster Life Cycle | Temperate |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Small pinhole-like plaques, that with enough time, develop translucent halos. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 1.6*10^11pfu/mL |
| Date of SEA Lysate Titering | Nov 3, 2016 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
