Mycobacterium phage Norbaka
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| Detailed Information for Phage Norbaka | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ellie Lunn and VyVy Moy |
| Year Found | 2023 |
| Location Found | Peabody, MA United States of America |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | The sample was dug in the backyard of a house in Peabody, MA. The sample was collected in front of the bush and between the two solar powered lights (Figure 1) The sample was collected by hand by turning a clean plastic ziploc bag inside out, putting it over the hand and grabbing a handful of the dirt. The bag was kept between my hand and the soil the entire time, then inverted the bag back to its original position while holding the sample still and sealed to keep the soil isolated. It was approximately 10:15 am on 9/19/23. It was around 70 degrees fahrenheit and sunny. The top of the soil was dry due to lack of rain, however the layers underneath where the sample was collected, about 6 inches down from the surface, were more moist as they were visually darker and softer to the touch. The sample was collected right before lab that day and was stored in just the bag, with unknown temperature for storage, for about 2 hours until used in the lab. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | All three plates were cloudy and had a cloudy ring around them. The 10-1 plate had some cloudy spots while the 10-3 plate had many spots that could be seen. From the plate we observed which was the 10-5 dilution plate, the phage we used was about 0.3 centimeters in diameter. There were at least 10 phages on this plate. All the phages and the plate itself were somewhat cloudy meaning that it was lysogenic. The plate appeared to be heterogeneous as some phages were different sizes than others. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 182 |
| Pitt Freezer Box Grid# | C4 |
| Available Files | |
| Plaque Picture | Download |
