Streptomyces phage PepetheSlayer
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| Detailed Information for Phage PepetheSlayer | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Jays,Alka & Telli,Ayo |
| Year Found | 2019 |
| Location Found | Lanham, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 38.957009 N, 76.852955 E Map |
| Discovery Notes | This soil sample was collected near the side of the road on a grassy area, a few centimeters deep. The temperature was cool and the soil was moist. |
| Naming Notes | We named our phage PepetheSlayer because the titer concentration our phage generated on S. mirbailis was so high!. Our highest calculation was about 2.8 x 10^12 pfu/mL. This means that PepetheSlayer can infact slay S. mirabilis bacteria! :) |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Singleton |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | Patch Assay, Liquid Phage Release and Sensitivity Assay were all conducted to test lysogeny. Results suggest bacteriophage is temperate and it's lysogens have the machinery for super infect immunity against our phage. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | We had two morphologies the first was described as small, circular and clear with an average diameter size of 3mm. Average plaque morphology was 9mm. The morphology was described as circular plaques having a dark turbid inner clearing surrounded by a lighter turbid halo with radial striations formed after 24hrs at 37 degrees Celsius. This morphology was first seen after purification 3 and then reoccurred during host range testing. A new lysate was also generated and all plaque morphologies for experiments using that lysate, were similar to the second mophology having radial striations. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.8 x 10^12 pfu/mL |
| Pitt Freezer Box# | 101 |
| Pitt Freezer Box Grid# | D2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
