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3' versus 5' overhangs in sequencing data
Dan Russell
|
Oct 31, 2014
Recently, Eric Miller at NC State asked me about phage genome ends, and how a 3' overhang (sticky end) would look in an assembled genome compared to how a 5' overhang would look. So I made a picture! Hopefully it explains how you might diagnose either of these cases from sequencing reads, and how you can distinguish between them.

Click the image below to view/download the full-size version.