As the last Question of Month for this school year, we would like you to think about how your analyses of your bacteriophage genome(s) can direct the kinds of questions you can ask about bacteriophages.

Using the genome(s) you have analyzed, …

The two photos shown here display a ten-fold dilution series of infection of 8 phages on M. smegmatis mc2 155 and M. smegmatis mc2 155 (Courthouse). M. smegmatis mc2155 (Courthouse) is a lysogen made with Mycobacteriophage Courthouse. How would you …

Scenario: The entire class of 20 students used the auto-annotation function of DNA Master to predict the genes of your class phage. Not all results returned the same number of genes and features? Why?
As you answer this question, please report …

Happy New Year! Before we move to the dark side of bacteriophages, the bioinformatic side, I thought one more question about plaque morphology could be appropriate. Here is a photo of plaques (on M. smeg) of Mycobacteriophage Spartacus that have …

To what extent do you think that the phages we have isolated on Mycobacterium smegmatis mc2 155 are reflective of the broader phage population?

In other words, during the process of phage discovery, enrichment, amplification, DNA extraction, and sending samples for …