The Actinobacteriophage Database

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Detailed Information for Phage Zelda
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Rohan Samarth
Year Found	2011
Location Found	St. Louis, MO USA
Finding Institution	Washington University in St. Louis
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	38.6435 N, 90.315167 W Map
Discovery Notes	The soil was slightly damp and around 13 cm deep. The air temperature was around 75°F and it was sunny. There was little foot traffic seen in the area or disturbance in the ground.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Oct 18, 2013
Sequencing Facility	The McDonnell Genome Institute at Washington University
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	262
Sanger Finishing Reactions	5
Genome length (bp)	68939
Character of genome ends	Circularly Permuted
GC Content	66.5%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	B
Subcluster	B1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View BirdsNest (None) Indlovu (None) LilMcDreamy (None) AbsoluteMadLad (B1) ABU (B1) AltPhacts (B1) Altwerkus (B1) Antonia (B1) Apizium (B1) Ashraf (B1) Badfish (B1) Banjo (B1) BatteryCK (B1) Beaglebox (B1) Bishoperium (B1) BlackStallion (B1) Buckeye (B1) CamL (B1) Cannibal (B1) Carthage (B1) Chah (B1) CharlieGBrown (B1) CheetO (B1) Childish (B1) Chorkpop (B1) Chunky (B1) Cobra (B1) Colbert (B1) Cosmolli16 (B1) Craff (B1) Crownjwl (B1) Daffy (B1) Derpp (B1) Dingo (B1) Dione (B1) Doddsville (B1) DoesntMatter (B1) DonSanchon (B1) DuchessDung (B1) Duggie (B1) Durga (B1) Emiris (B1) EmpTee (B1) Eremos (B1) Eugenia (B1) Fang (B1) FluffyNinja (B1) FriarPreacher (B1) Fringe (B1) FugateOSU (B1) Gareth (B1) GeneCoco (B1) Gophee (B1) Grand2040 (B1) Gyarad (B1) Haimas (B1) Haleema (B1) Hamish (B1) Harvey (B1) Held (B1) HenryJackson (B1) Hertubise (B1) Hetaeria (B1) HighStump (B1) Hocus (B1) Horchata (B1) HSavage (B1) ImtiyazSitla (B1) Iridoclysis (B1) IsaacEli (B1) JacAttac (B1) JakeO (B1) JangoPhett (B1) Jillium (B1) Jiminy (B1) Kahve (B1) Kailash (B1) Keitherie (B1) Kikipoo (B1) KingTut (B1) KingVeVeVe (B1) KlimbOn (B1) Kloppinator (B1) KLucky39 (B1) Kwadwo (B1) Kwksand96 (B1) Labeouficaum (B1) Lasso (B1) LeeLot (B1) Lego3393 (B1) Legolas (B1) LemonSlice (B1) Longacauda (B1) LuckyMarjie (B1) Lulumae (B1) Mag7 (B1) Mana (B1) Manad (B1) Maskar (B1) Matalotodo (B1) Mcshane (B1) Mecca (B1) Megatron (B1) Mesh1 (B1) MichaelPhcott (B1) Mikota (B1) MitKao (B1) Morgushi (B1) Morty (B1) Mosaic (B1) MRabcd (B1) Mulan (B1) Murdoc (B1) Mutante (B1) Nacho (B1) Newman (B1) Numberten (B1) Oline (B1) Olive (B1) OliverWalter (B1) Omniscient (B1) Oosterbaan (B1) Orion (B1) OSmaximus (B1) PG1 (B1) Phamished (B1) Phareon (B1) PhatCats2014 (B1) PhenghisKhan (B1) Phergie (B1) Phipps (B1) Phleuron (B1) PhrankReynolds (B1) PhrodoBaggins (B1) Phunky (B1) Piglet (B1) PinheadLarry (B1) Pinkman (B1) Pipsqueak (B1) Placalicious (B1) Plmatters (B1) Podrick (B1) Pops (B1) Potter (B1) Prickles (B1) ProfessorX (B1) Puhltonio (B1) QueenBeane (B1) RedMaple (B1) Riggan (B1) Robyn (B1) Roliet (B1) Roscoe (B1) Roy17 (B1) Samaymay (B1) SassyCat97 (B1) Schadenfreude (B1) Scoot17C (B1) SDcharge11 (B1) Serendipity (B1) Serpentine (B1) Sheila (B1) ShiVal (B1) Sigman (B1) Skippy (B1) Solosis (B1) Sophia (B1) Soto (B1) Spartan300 (B1) Squid (B1) Squiggle (B1) Struggle (B1) Suffolk (B1) Surely (B1) Swish (B1) TallGrassMM (B1) Thora (B1) ThreeOh3D2 (B1) Timmi (B1) TomBombadil (B1) Trypo (B1) TyrionL (B1) UAch1 (B1) UncleHowie (B1) Vaishali24 (B1) Valjean (B1) Vaticameos (B1) Veritas (B1) Virapocalypse (B1) Vista (B1) Vivaldi (B1) Vortex (B1) Wallhey (B1) Waterdiva (B1) Xavier (B1) Yoshand (B1) YouGoGlencoco (B1) Zaider (B1) Zelda (B1) Zonia (B1) Arbiter (B2) Ares (B2) Bananafish (B2) Boyle (B2) Brownie5 (B2) Coffee (B2) Eaglehorse (B2) Faze9 (B2) FrenchFry (B2) Glass (B2) Godines (B2) Hedgerow (B2) Holeinone (B2) ItsyBitsy1 (B2) Kheth (B2) Laurie (B2) Lephleur (B2) LizLemon (B2) Opia (B2) Qyrzula (B2) Rhinoforte (B2) Rosebush (B2) Sabella (B2) TA17a (B2) Tinciduntsolum (B2) Tres (B2) West99 (B2) Abinghost (B3) Akoma (B3) Athena (B3) Audrey (B3) Baloo (B3) Bernardo (B3) ChaChing (B3) Chandler (B3) Compostia (B3) Corofin (B3) Daisy (B3) Gadjet (B3) Heathcliff (B3) Jackstina (B3) Kamiyu (B3) Marley1013 (B3) Mortcellus (B3) Morty007 (B3) Nozo (B3) Obutu (B3) OrangeOswald (B3) Phaedrus (B3) Philly (B3) Phlyer (B3) Pipefish (B3) RagingRooster (B3) Rita1961 (B3) RomaT (B3) SynergyX (B3) Tydolla (B3) Yahalom (B3) AlanGrant (B4) Apex (B4) BrownCNA (B4) ChrisnMich (B4) Cooper (B4) Fortunato (B4) Frederick (B4) Hangman (B4) JAMaL (B4) Lolalove (B4) Magpie (B4) Mithril (B4) Nigel (B4) RawrgerThat (B4) Stinger (B4) Vincenzo (B4) Waleliano (B4) Zemanar (B4) Acadian (B5) Baee (B5) Donny (B5) Phelemich (B5) Reprobate (B5) Rich (B5) Serendipitous (B5) 39HC (B6) 40BC (B6) Hosp (B6) Jolie1 (B6) KayaCho (B6) Saguaro (B7) Thonko (B8) Imvubu (B9)
Annotating Institution	University of Pittsburgh
Annotation Status	In GenBank
Plaque Notes	average plaque size of 3.0mm. There was slight variation and slightly fuzzy around the edges, however there were no visible haloes. The plaques showed no turbidity.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Zelda_1 (adenylate kinase) Zelda_2 (terminase) Zelda_3 Zelda_4 Zelda_5 Zelda_6 (RuvC-like resolvase) Zelda_7 Zelda_8 (portal protein) Zelda_9 (capsid maturation protease and MuF-like fusion protein) Zelda_10 (HNH endonuclease domain protein) Zelda_11 Zelda_12 (major capsid protein) Zelda_13 Zelda_14 Zelda_15 (membrane protein) Zelda_16 (membrane protein) Zelda_17 (membrane protein) Zelda_18 (major tail protein) Zelda_19 Zelda_20 (queuine tRNA-ribosyltransferase) Zelda_21 Zelda_22 (head-to-tail adaptor) Zelda_23 Zelda_24 Zelda_25 (tail assembly chaperone) Zelda_26 Zelda_27 Zelda_28 (tape measure protein) Zelda_29 (minor tail protein) Zelda_30 (minor tail protein) Zelda_31 (minor tail protein) Zelda_32 (minor tail protein) Zelda_33 (minor tail protein) Zelda_34 Zelda_35 Zelda_36 Zelda_37 Zelda_38 Zelda_39 Zelda_40 Zelda_41 Zelda_42 Zelda_43 Zelda_44 Zelda_45 (helix-turn-helix DNA binding domain protein) Zelda_46 (helix-turn-helix DNA binding domain protein) Zelda_47 Zelda_48 (lysin A) Zelda_49 (lysin B) Zelda_50 Zelda_51 Zelda_52 (DNA helicase) Zelda_53 Zelda_54 Zelda_55 Zelda_56 Zelda_57 Zelda_58 (DNA primase/polymerase) Zelda_59 Zelda_60 Zelda_61 (DNA polymerase I) Zelda_62 Zelda_63 Zelda_64 Zelda_65 Zelda_66 Zelda_67 Zelda_68 Zelda_69 Zelda_70 (helix-turn-helix DNA binding protein) Zelda_71 Zelda_72 Zelda_73 Zelda_74 Zelda_75 (HNH endonuclease) Zelda_76 Zelda_77 Zelda_78 Zelda_79 Zelda_80 Zelda_81 Zelda_82 Zelda_83 Zelda_84 (HNH endonuclease) Zelda_85 Zelda_86 Zelda_87 Zelda_88 Zelda_89 Zelda_90 Zelda_91 Zelda_92 Zelda_93 Zelda_94 Zelda_95 Zelda_96 Zelda_97 Zelda_98 (membrane protein) Zelda_99 Zelda_100 Zelda_101 Zelda_102
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MK112555
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2011WUSLzeldaKH
SEA Lysate Titer	2.12e10
Date of SEA Lysate Titering	Oct 4, 2011
Pitt Freezer Box#	31
Pitt Freezer Box Grid#	7
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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