| Detailed Information for Phage Amigo | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Yasmene Warrad |
| Year Found | 2013 |
| Location Found | Spring, TX USA |
| Finding Institution | Baylor University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 30.023958 N, 95.563788 W Map |
| Discovery Notes | I isolated from a garden bed about 20 cm deep and it was approximately 98 degrees. |
| Naming Notes | Named after a Chihuahua I dog-sit. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 19, 2014 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 |
| Sequencer Used | 454 Genome Sequencer FLX |
| Approximate Shotgun Coverage | 776 |
| Sanger Finishing Reactions | 4 |
| Genome length (bp) | 59173 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1584 bp |
| GC Content | 52.9% |
| Sequencing Notes | One end (the right end) of the genome is clear from the buildup of reads after shotgun sequencing. The other end is less clear. We chose a base near the left side of a double coverage region that usually indicates a terminal repeat, but the buildup of reads at that base was not nearly as clear as at the genome's right end. With the chosen base, the genome has a terminal repeat of 1584 bp, though this is an approximation and it's likely that not every particle has that precise left end. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AQ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Lytic center with a huge halo around it. |
| Morphotype | Siphoviridae |
| Number of Genes | 86 |
| Number of tRNAs | 7 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewAmigo_1 Amigo_2 Amigo_3 Amigo_4 Amigo_5 Amigo_6 Amigo_7 Amigo_8 Amigo_9 Amigo_10 Amigo_11 Amigo_12 — tRNA Amigo_13 — tRNA Amigo_14 — tRNA Amigo_15 — tRNA Amigo_16 — tRNA Amigo_17 Amigo_18 Amigo_19 Amigo_20 Amigo_21 Amigo_22 Amigo_23 Amigo_24 Amigo_25 Amigo_26 Amigo_27 Amigo_28 Amigo_29 Amigo_30 Amigo_31 Amigo_32 Amigo_33 Amigo_34 Amigo_35 Amigo_36 Amigo_37 Amigo_38 Amigo_39 Amigo_40 Amigo_41 Amigo_42 Amigo_43 Amigo_44 Amigo_45 Amigo_46 Amigo_47 Amigo_48 Amigo_49 Amigo_50 Amigo_51 Amigo_52 Amigo_53 Amigo_54 Amigo_55 Amigo_56 Amigo_57 Amigo_58 — tRNA Amigo_59 — tRNA Amigo_60 Amigo_61 Amigo_62 Amigo_63 Amigo_64 Amigo_65 Amigo_66 Amigo_67 Amigo_68 Amigo_69 Amigo_70 Amigo_71 Amigo_72 Amigo_73 Amigo_74 Amigo_75 Amigo_76 Amigo_77 Amigo_78 Amigo_79 Amigo_80 Amigo_81 Amigo_82 Amigo_83 Amigo_84 Amigo_85 Amigo_86 Amigo_87 Amigo_88 Amigo_89 Amigo_90 Amigo_91 Amigo_92 Amigo_93 |
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU160638 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.0*10^11 |
| Date of SEA Lysate Titering | Nov 6, 2013 |
| Pitt Freezer Box# | 147 |
| Pitt Freezer Box Grid# | F6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
