| Detailed Information for Phage Boise | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Disinphectant, AttackonPhages, Phushiguro, Phree99 |
| Year Found | 2025 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.06532 N, 118.45183 W Map |
| Discovery Notes | Soil was moist and found near the sidewalk of a suburban area. The location was well-shaded with small shrubbery nearby. Depth from which sample was taken (in cm): 3 cm Air temperature if sample collected outdoors (in degrees Celsius): 20.5 degrees celsius Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. AL Quarter and Lab section: F25-1C Instructor: Dr. Tejas Bouklas Teaching Assistant: Usha N. Undergraduate Assistant(s): Destiny, Rita Team #1 Name: Attack on Phages Team #1 Members: Nathan, Aidan, Liana, Bryant, Kayla Team #2 Name: DisinPHectant Team #2 Members: Sahana, Megan, Gabriela, Avantika Team #3 Name: Phushiguro Team #3 Members: Aditya Patki, Archen Panganiban, Julia Yoho, Katirina Keo Team #4 Name: Phree.99 Team #4 Members: Alyssa Manzur, Angelica Malau, Princess Jereza, Sarah Sun |
| Naming Notes | Boise, Idaho- make our small town dreams come true |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 151 |
| Genome length (bp) | 61751 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1042 bp |
| GC Content | 60.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FT |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Pinpoint, halo and bullseye before purifying. Bullseye/turbid after purification Description of plaque morphology: Small, turbid, bullseye after purification Average size (and size range) diameter in mm: 0.5mm |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBoise_1 Boise_2 Boise_3 Boise_4 Boise_5 Boise_6 Boise_7 Boise_8 Boise_9 Boise_10 Boise_11 Boise_12 Boise_13 Boise_14 Boise_15 Boise_16 Boise_17 Boise_18 Boise_19 Boise_20 Boise_21 Boise_22 Boise_23 Boise_24 Boise_25 Boise_26 Boise_27 Boise_28 Boise_29 Boise_30 Boise_31 Boise_32 Boise_33 Boise_34 Boise_35 Boise_36 Boise_37 Boise_38 Boise_39 Boise_40 Boise_41 Boise_42 Boise_43 Boise_44 Boise_45 Boise_46 Boise_47 Boise_48 Boise_49 Boise_50 Boise_51 Boise_52 Boise_53 Boise_54 Boise_55 Boise_56 Boise_57 Boise_58 Boise_59 Boise_60 Boise_61 Boise_62 Boise_63 Boise_64 Boise_65 Boise_66 Boise_67 Boise_68 Boise_69 Boise_70 Boise_71 Boise_72 Boise_73 Boise_74 Boise_75 Boise_76 Boise_77 Boise_78 Boise_79 Boise_80 Boise_81 Boise_82 Boise_83 Boise_84 Boise_85 Boise_86 Boise_87 Boise_88 Boise_89 Boise_90 Boise_91 Boise_92 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |