Arthrobacter phage Bolt007
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Detailed Information for Phage Bolt007
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByKelley Krug
Year Found2021
Location FoundMalibu, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.047777 N, 118.578333 W Map
Discovery NotesSoil setting:
Urban, couple trees, a cactus, shrubs and brush, 40 ft from a mountainous road, sloped, northeast-facing
Depth from which sample was taken: 12cm
Air temperature: 30

AL Quarter and Lab section: F21-1B
Instructor: Amanda Freise; Ana Garcia Vedrenne
Teaching Assistant: Ryan Ngo
Undergraduate Assistant(s): Amanda Chai

Team #1 Name: Phage on the Run
Team #1 Members: Minehli Kamarzar, Kelley Krug, Maysaa Batteikh, Manar Abuwarda
Naming NotesOur team name was Phage on the Run so we decided to name our phage Bolt007 after the movie characters Bolt and James Bond, since they’re both spies on the run.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 4, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2108
Genome length (bp)49798
Character of genome endsCircularly Permuted
GC Content69.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterFH
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesHalo plaques: clear with turbid ring on outside
~1mm on average
MorphotypeMyoviridae
Number of Genes78
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Bolt007_1
(terminase small subunit)

Bolt007_2
(terminase large subunit)

Bolt007_3
(portal protein)

Bolt007_4
(capsid maturation protease)

Bolt007_5

Bolt007_6
(scaffolding protein)

Bolt007_7
(head fiber protein)

Bolt007_8
(major capsid protein)

Bolt007_9

Bolt007_10

Bolt007_11

Bolt007_12

Bolt007_13

Bolt007_14
(tail sheath protein)

Bolt007_15
(tail tube protein)

Bolt007_16
(tail assembly chaperone)

Bolt007_17

Bolt007_18
(tape measure protein)

Bolt007_19
(LysM-like peptidoglycan binding protein)

Bolt007_20
(minor tail protein)

Bolt007_21

Bolt007_22
(minor tail protein)

Bolt007_23

Bolt007_24
(minor tail protein)

Bolt007_25

Bolt007_26

Bolt007_27

Bolt007_28
(endolysin)

Bolt007_29

Bolt007_30

Bolt007_31
(baseplate wedge protein)

Bolt007_32
(baseplate J protein)

Bolt007_33
(minor tail protein)

Bolt007_34
(minor tail protein)

Bolt007_35

Bolt007_36

Bolt007_37
(RecE-like exonuclease)

Bolt007_38
(RecT-like DNA pairing protein)

Bolt007_39

Bolt007_40
(helix-turn-helix DNA binding domain protein)

Bolt007_41

Bolt007_42

Bolt007_43
(ssDNA binding protein)

Bolt007_44
(RepA-like replication initiator)

Bolt007_45
(helicase loader)

Bolt007_46

Bolt007_47

Bolt007_48

Bolt007_49

Bolt007_50
(RusA-like resolvase)

Bolt007_51

Bolt007_52

Bolt007_53
(helix-turn-helix DNA binding domain protein)

Bolt007_54

Bolt007_55

Bolt007_56

Bolt007_57

Bolt007_58

Bolt007_59

Bolt007_60

Bolt007_61

Bolt007_62
(ParB-like nuclease domain protein)

Bolt007_63

Bolt007_64

Bolt007_65
(oxidoreductase)

Bolt007_66
(glycosyltransferase)

Bolt007_67
(glucosaminyl deacetylase)

Bolt007_68

Bolt007_69

Bolt007_70

Bolt007_71
(helix-turn-helix DNA binding domain protein)

Bolt007_72

Bolt007_73

Bolt007_74

Bolt007_75

Bolt007_76

Bolt007_77

Bolt007_78

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOP985600
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 139
Pitt Freezer Box Grid# F8
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload