Mycobacterium phage Bonk
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| Detailed Information for Phage Bonk | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Connor Flick |
| Year Found | 2020 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.989861 N, 86.451028 W Map |
| Discovery Notes | Taken on: 8-23-2020, 2:00 p.m. Depth of soil: ~8cm Temperature: 82°F Weather: Sunny, without clouds. Soil Conditions: The sample was under a large amount of mulch, some of which was inadvertently collected with the sample. Generally soft, clumped together, and gave easily when dug into. Sample was moist, leaving moisture on my hand when examined. Texture felt analogous to a sponge or cake; firm but compressible. Found beside a Chili's; 268 Alumni Ave, Bowling Green, KY 42101 The phage was isolated through a series of serial dilutions of the enrichment, which ended at a 10^-6 dilution. The plaque formed on the 10^-6 dilution's plate was by itself and naturally purified. Purification was confirmed through a dilution of the plaque yielding no changes in morphology. |
| Naming Notes | Bonk is a short, simplistic, and mildly amusing word that has been used more and more throughout the world and within my own life. As such, it felt right to name a phage after it. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques of Bonk are clear circles without any noticeable turbidity inside or around the plaque. Plaques average 2.5mm but can range from 1.5 to 4mm depending on concentration. There has been no noticeable variation in the sizes or turbidity of the plaques since the purification of Bonk. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.8 * 10^9 |
| Pitt Freezer Box# | 120 |
| Pitt Freezer Box Grid# | D6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
