Mycobacterium phage BoopleSnoot
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| Detailed Information for Phage BoopleSnoot | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Makenna Kayser |
| Year Found | 2024 |
| Location Found | Holland, MI United States of America |
| Finding Institution | Hope College |
| Program | Unavailable |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 48.029011 N, 98.121223 E Map |
| Discovery Notes | sample collected on August 27, 2024 at 8:20 pm (EST) Temperature: 80°F soil was dry, soil was collected at a depth of 9.5 mm soil was from under my families (dying) lily bed |
| Naming Notes | My sister and I would always say “boop the snoot” when we would poke our pets’ noses, and once I slipped up and said “BoopleSnoot” it's been a running joke ever since. Also, I couldn't stop laughing at the thought of a microbiologist trying to give a serious lecture while also having to say BoopleSnoot several times throughout |
| Sequencing Information | |
| Sequencing Complete? | No |
| Date Sequencing Completed | Dec 23, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 3628 |
| Genome length (bp) | 53371 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 14 bases |
| Overhang Sequence | TAAGCCGCGCGGTA |
| GC Content | 67.5% |
| Fasta file available? | No |
| Characterization | |
| Cluster | Q |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Annotating Institution | Hope College |
| Annotation Status | Not sequenced |
| Plaque Notes | After ≈48 hours of incubation at 35°C the plaques are clear and have multiple sizes ranging from ≈1-3mm. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 196 |
| Pitt Freezer Box Grid# | G6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
