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Mycobacterium phage Charlie
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Detailed Information for Phage Charlie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySanjeev Chalissery, Neil Mistry
Year Found2009
Location FoundRichmond, VA USA
Finding InstitutionVirginia Commonwealth University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.544958 N, 77.454041 W Map
Discovery Notes75 deg F, collected under a cherry tree in mulched soil, 2 cm
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 22, 2011
Genome length (bp)43036
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCAATGG
GC Content66.3%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesPlaques aren't completely round. ~1.5mm diameter, clear with turbid edge.
Number of Genes68
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJN256079
Refseq NumberNC_023729
Published in PaperHatfull et al 2012, Journal of Virology: Complete genome sequences of 138 mycobacteriophages
Archiving Info
Archiving status Archived
SEA Designator 2009VACUCharlieSCNM
SEA Lysate Titer 3.60e11
Date of SEA Lysate Titering Nov 15, 2009
Pitt Freezer Box# 1
Pitt Freezer Box Grid# D8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload