Mycobacterium phage Borgus
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| Detailed Information for Phage Borgus | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Davis Elliott |
| Year Found | 2015 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.986167 N, 86.454417 W Map |
| Discovery Notes | The sample from which the bacteriophage Borgus was isolated was gathered on September 1, 2015 at 12:43 pm. The temperature was 31°C, with 46% humidity. The soil was dry, but not dusty or hard. The site is surrounded by many plants and is the shade of several trees. The bacteriophage was isolated from within the top 7cm of soil. |
| Naming Notes | ***Bulls-eye mutant present (Borgus-Bullseye)*** |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | The titre of the 10-plate lysate was 4.55 x 10^8 pfu/mL. Borgus was analyzed with a spectrophotometer in order to calculate the DNA concentration. The DNA concentration was recorded as 68.4 μg/mL. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Borgus grows in turbid circles, ranging from pin-points to a couple millimeters large. The plaques are often very hard to see without holding up to the light. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2015WEKUborgusDLE |
| SEA Lysate Titer | 4.55 x 10^8 |
| Date of SEA Lysate Titering | Nov 9, 2015 |
| Pitt Freezer Box# | 27 |
| Pitt Freezer Box Grid# | D12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
