| Detailed Information for Phage ZygoTaiga |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Carmen Aguirre |
| Year Found | 2012 |
| Location Found | Duluth, MN USA |
| Finding Institution | College of St. Scholastica |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 46.8 N, 92.08 W Map |
| Discovery Notes | The phage we isolated was from a park in down town duluth. The location was surrounded by streets on all sides and had a playground within 300 feet. It was a grassy, and damp area near trees. I isolated the phage around 12:00am; the night was cloudy, foggy and colder (around 40*F) which made the soil sample very damp. |
| Naming Notes | A blend of the Taiga forest near where I found my phage and zygote cause our phage was one of a kind. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 12, 2013 |
| Sequencing Facility | Virginia Commonwealth University Nucleic Acids Research Facilities |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 110 |
| Sanger Finishing Reactions | 2 |
| Genome length (bp) | 157204 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Our phage that we isolated are very small, clear plaques and this shows to be very consistent throughout our purification and empirical testing. From my observations in the past 2 months I have come to a conclusion that my plaque seems to be a lytic phage (of course there is no certainty in that but it has shown no expression of temperate characteristics). Our phage is very slow growing and can be somewhat difficult to work with, but our plates have come out very consistent. |
| Morphotype | Myoviridae |
| Number of Genes | 232 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KM881426 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
34 |
| Pitt Freezer Box Grid# |
53 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
