Mycobacterium phage BubbleBuddy
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| Detailed Information for Phage BubbleBuddy | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Krishna Patel |
| Year Found | 2017 |
| Location Found | Bensalem, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 9°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | The location where this sample was extracted was in suburban Pennsylvania in Bensalem, approximately twenty minutes from the city of Philadelphia. The sample was extracted from a garden bed/vegetable garden, approximately 2.54 cm in depth into the soil. Although the temperature of the soil sample is unknown, the sample was collected in colder temperatures. The temperature outside was approximately 48 degrees Fahrenheit or approximately 8.9 or 9 degrees Celsius. The sample was collected in an open location with nothing in its proximity to affect the soil's characteristics. However, it rained the day of collection, as well as the previous day, making the environmental sample wet during collection. |
| Naming Notes | This name was chosen due to the morphology of the plaques. The plaques were clear and formed in the form of a bubble. Therefore, I named my phage after the character in Spongebob: Bubble Buddy. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | University of Pittsburgh Genomics and Proteomics Core Laboratories |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Throughout purification and other plating procedures, the plaques were clear with no presence of any turbidity. The plaque was clear throughout and was approximately 1mm in diameter. The plaque was completely clear with no presence of a turbid ring around the plaque. Therefore, the plaque has no turbidity. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3x10 pfu/ml |
| Date of SEA Lysate Titering | Mar 30, 2017 |
| Pitt Freezer Box# | 51 |
| Pitt Freezer Box Grid# | C3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
