| Detailed Information for Phage C3PO | |
| Discovery Information | |
| Isolation Host | Corynebacterium vitaeruminis NCIB 9291 |
| Found By | Brittaney Johnson and Darshan Patel |
| Year Found | 2015 |
| Location Found | Birmingham, AL USA |
| Finding Institution | University of Alabama at Birmingham |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.44192024533113 N, 86.8248219 W Map |
| Discovery Notes | Original sample was sewage influent from a Jefferson County sewage treatment plant. Sample was first filtered through a 0.2uM filter in a biosafety cabinet, then enriched for 48 hours, 30C, 225rpm. Sample was then filtered again through 0.2uM filter in a biosafety cabinet. Filtered enrichment sample then spotted for presence of phage infecting Corynebacterium. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1180 |
| Genome length (bp) | 67383 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CCTACGGTGC |
| GC Content | 52.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EN |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to View |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques not visible at 24 hour incubation at 30C. Plaques visible at 48 hour incubation. Plaque size variable (1-2mm in diamater). |
| Number of Genes | 93 |
| Number of tRNAs | 4 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewC3PO_1 C3PO_2 C3PO_3 C3PO_4 C3PO_5 C3PO_6 C3PO_7 C3PO_8 C3PO_9 C3PO_10 C3PO_11 C3PO_12 C3PO_13 C3PO_14 C3PO_15 C3PO_16 C3PO_17 C3PO_18 C3PO_19 C3PO_20 C3PO_21 C3PO_22 C3PO_23 C3PO_24 C3PO_25 C3PO_26 C3PO_28 C3PO_27 C3PO_29 C3PO_30 C3PO_31 C3PO_32 C3PO_33 C3PO_34 C3PO_35 C3PO_36 C3PO_37 C3PO_38 C3PO_39 C3PO_40 C3PO_41 C3PO_42 C3PO_43 C3PO_44 C3PO_45 C3PO_46 — tRNA C3PO_47 — tRNA C3PO_48 — tRNA C3PO_49 — tRNA C3PO_50 C3PO_51 C3PO_52 C3PO_53 C3PO_54 C3PO_55 C3PO_56 C3PO_57 C3PO_58 C3PO_59 C3PO_60 C3PO_61 C3PO_62 C3PO_63 C3PO_64 C3PO_65 C3PO_66 C3PO_67 C3PO_68 C3PO_69 C3PO_70 C3PO_71 C3PO_72 C3PO_73 C3PO_74 C3PO_75 C3PO_76 C3PO_77 C3PO_78 C3PO_79 C3PO_80 C3PO_81 C3PO_82 C3PO_83 C3PO_84 C3PO_85 C3PO_86 C3PO_87 C3PO_88 C3PO_89 C3PO_90 C3PO_91 C3PO_92 C3PO_93 C3PO_94 C3PO_95 C3PO_96 C3PO_97 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG198776 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.0x10^9 |
| Pitt Freezer Box# | 26 |
| Pitt Freezer Box Grid# | D9 |
| Available Files | |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |