| Detailed Information for Phage CallinAllBarbz | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Daniel Chen |
| Year Found | 2022 |
| Location Found | Altadena, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.196944 N, 118.102222 W Map |
| Discovery Notes | Isolated by TA Dominic Garza. Mixed culture used for isolation: An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method. Sample description: The soil sample was moist. The collection site was rural and far away from any buildings; however, there was quite a lot of foot traffic considering it was taken from along the Eaton Canyon hiking trail. There was a waterfall and stream close to the soil collection site. |
| Naming Notes | Barbz is a nickname for Nicki Minaj’s online army of devoted fans. The Barbz are the literal embodiment of both fearlessness and drive. CallinAllBarbz pays homage to Queen Nicki and her Barbz. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 2, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1573 |
| Genome length (bp) | 41205 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCACCGGCA |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FP |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Description of plaque morphology: Bullseye with turbid center and clear edges. Average diameter: 10 mm |
| Number of Genes | 62 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewCallinAllBarbz_1 CallinAllBarbz_2 CallinAllBarbz_3 CallinAllBarbz_4 CallinAllBarbz_5 CallinAllBarbz_6 CallinAllBarbz_7 CallinAllBarbz_8 CallinAllBarbz_9 CallinAllBarbz_10 CallinAllBarbz_11 CallinAllBarbz_12 CallinAllBarbz_13 CallinAllBarbz_14 CallinAllBarbz_15 CallinAllBarbz_16 CallinAllBarbz_17 CallinAllBarbz_18 CallinAllBarbz_19 CallinAllBarbz_20 CallinAllBarbz_21 CallinAllBarbz_22 CallinAllBarbz_23 CallinAllBarbz_24 CallinAllBarbz_25 CallinAllBarbz_26 CallinAllBarbz_27 CallinAllBarbz_28 CallinAllBarbz_29 CallinAllBarbz_30 CallinAllBarbz_31 CallinAllBarbz_32 CallinAllBarbz_33 CallinAllBarbz_34 CallinAllBarbz_35 CallinAllBarbz_36 CallinAllBarbz_37 CallinAllBarbz_38 CallinAllBarbz_39 CallinAllBarbz_40 CallinAllBarbz_41 CallinAllBarbz_42 CallinAllBarbz_43 CallinAllBarbz_44 CallinAllBarbz_45 CallinAllBarbz_46 CallinAllBarbz_47 CallinAllBarbz_48 CallinAllBarbz_49 CallinAllBarbz_50 CallinAllBarbz_51 CallinAllBarbz_52 CallinAllBarbz_53 CallinAllBarbz_54 CallinAllBarbz_55 CallinAllBarbz_56 CallinAllBarbz_57 CallinAllBarbz_58 CallinAllBarbz_59 CallinAllBarbz_60 CallinAllBarbz_61 — tRNA CallinAllBarbz_62 — tRNA CallinAllBarbz_63 CallinAllBarbz_64 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR553891 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 155 |
| Pitt Freezer Box Grid# | G9 |
| Available Files | |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |