Arthrobacter phage CallinAllBarbz
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| Detailed Information for Phage CallinAllBarbz | |
| Discovery Information | |
| Isolation Host | Arthrobacter sulfureus ATCC 19098 |
| Found By | Daniel Chen |
| Year Found | 2022 |
| Location Found | Altadena, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.196944 N, 118.102222 W Map |
| Discovery Notes | Isolated by TA Dominic Garza. Mixed culture used for isolation: An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method. Sample description: The soil sample was moist. The collection site was rural and far away from any buildings; however, there was quite a lot of foot traffic considering it was taken from along the Eaton Canyon hiking trail. There was a waterfall and stream close to the soil collection site. |
| Naming Notes | Barbz is a nickname for Nicki Minaj’s online army of devoted fans. The Barbz are the literal embodiment of both fearlessness and drive. CallinAllBarbz pays homage to Queen Nicki and her Barbz. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 2, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1573 |
| Genome length (bp) | 41205 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTCACCGGCA |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | FP |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Description of plaque morphology: Bullseye with turbid center and clear edges. Average diameter: 10 mm |
| Number of Genes | 62 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR553891 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 155 |
| Pitt Freezer Box Grid# | G9 |
| Available Files | |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |