Arthrobacter phage CallinAllBarbz
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Detailed Information for Phage CallinAllBarbz
Discovery Information
Isolation HostArthrobacter sulfureus NRRL B-14730
Found ByDaniel Chen
Year Found2022
Location FoundAltadena, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.196944 N, 118.102222 W Map
Discovery NotesIsolated by TA Dominic Garza.

Mixed culture used for isolation:
An enriched culture was prepared by combining 15 mL of soil with 25 mL of PYCA broth. The bacterial strains of A. sp., A. globiformis, A. sulfureus and A. atrocyaneus were then added in a ratio of 1:5:192:588 respectively. The enriched culture was incubated at 30℃ for 48 hours with shaking. After filtering with a 0.22 µm filter, the sample was plated on PYCA plates with 2X PYCa soft agar using the standard pour plate method.

Sample description: The soil sample was moist. The collection site was rural and far away from any buildings; however, there was quite a lot of foot traffic considering it was taken from along the Eaton Canyon hiking trail. There was a waterfall and stream close to the soil collection site.
Naming NotesBarbz is a nickname for Nicki Minaj’s online army of devoted fans. The Barbz are the literal embodiment of both fearlessness and drive. CallinAllBarbz pays homage to Queen Nicki and her Barbz.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 2, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1573
Genome length (bp)41205
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCTCACCGGCA
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterFP
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members

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Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusBeing Annotated (Expected completion by 3/2/2023)
Plaque NotesDescription of plaque morphology: Bullseye with turbid center and clear edges.
Average diameter: 10 mm
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload