Mycobacterium phage CrazyJacki
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Detailed Information for Phage CrazyJacki
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesJackiiscrazy
Found ByJacki Ayotte and Jordan Coleman
Year Found2023
Location FoundNorth Andover, MA United States of America
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates42.66901 N, 71.12352 W Map
Discovery NotesThe samples were collected in a plastic vial and gloves were used to prevent
contamination. The temperature outside was approximately 18 degrees C and the samples were collected at 9:45 am. The vials of the samples were then placed in a plastic bag and brought into the laboratory. The third sample was a soil sample taken by the Merrimack College Pond on the outside, where the soil and water are separated from each other by a wall. All samples were taken approximately 30 minutes before isolation.
Naming NotesNamed after the student who discovered the phage who may or may not actually be crazy.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe size of this plaque was roughly 0.5cm, and about ten phages were clear and the rest were cloudy and had different sizes, meaning the plate overall had a heterogeneous mixture of plaques. The figure titled “Figure 2” below shows the one plate used, and the black dot on the picture is the exact plaque used for the following generation. This plate did not have any cloudy edges. The reasoning to choosing the plate labeled 10-1 was because the
other two plates did not have any plaques.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 182
Pitt Freezer Box Grid# B7
Available Files
Plaque PictureDownload